Cross-reactivity test of sandwich ELISA.

<p>Four other genera of bacterial pathogens and 19 serotypes of <i>E</i>.<i>coli</i> were evaluated with DAS-ELISA, and only EHEC O157:H7 had a positive value based on a threshold of OD₄₅₀. When OD₄₅₀ > 0.231, samples were positive, when OD450 ≤0.231, values were negative and not cross-reactive with EHEC O157:H7. All experiments were conducted in triplicate. Data are expressed as means ± SD.