<p>DAS-ELISA had a detection limit of 1 × 10<sup>3</sup> CFU/mL (OD<sub>450</sub> = 0.26, column 3) for EHEC O157:H7 pure culture (A) and 1 × 10<sup>4</sup> CFU/g (OD450 = 0.24, column 4) for artificial samples before enrichment (B). All experiments were conducted in triplicate. Data are expressed as average ± SD.</p
Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens...
Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens...
<p>A series of primary antibody dilutions (1:500, 1:600, 1:700, 1:800, 1:900 and 1:1000) in PBS were...
<p>Four other genera of bacterial pathogens and 19 serotypes of <i>E</i>.<i>coli</i> were evaluated ...
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen of worldwide importance tha...
<p>A) Optimizing sandwich ELISA antibody combinations. Antibodies were used at 1 μg/mL for capture a...
<p>In the sandwich ELISA assay format, the wells were coated with E1 mG2a/hG3 as a capture. Ten diff...
<p>Dilutions: 1∶1 = 4 microg/mL; 1∶10 = 400 ng/mL; 1∶10<sup>2</sup> = 40 ng/mL; 1∶10<sup>3</sup> = 4...
<p>Different ELISA setups (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.011...
<p>The response of the ELISA Systems sandwich assays to a commercial lager, (Tanner this journal, th...
<p>The scheme introduces the six sandwich ELISA protocols that were compared in the study.</p
<p>The detection of NS1 in culture supernatants of WNV-infected cells by different MAb pairs. The cu...
<p>10<sup>6</sup> yeast displaying the indicated antibodies were induced, washed and resuspended in ...
<p>(A) A group of typical signal-time traces at different concentrations of <i>E. coli</i> O157:H7 d...
<p>The response of ELISA Systems sandwich assay containing total Sloop hordein (500 ppb) and either ...
Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens...
Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens...
<p>A series of primary antibody dilutions (1:500, 1:600, 1:700, 1:800, 1:900 and 1:1000) in PBS were...
<p>Four other genera of bacterial pathogens and 19 serotypes of <i>E</i>.<i>coli</i> were evaluated ...
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen of worldwide importance tha...
<p>A) Optimizing sandwich ELISA antibody combinations. Antibodies were used at 1 μg/mL for capture a...
<p>In the sandwich ELISA assay format, the wells were coated with E1 mG2a/hG3 as a capture. Ten diff...
<p>Dilutions: 1∶1 = 4 microg/mL; 1∶10 = 400 ng/mL; 1∶10<sup>2</sup> = 40 ng/mL; 1∶10<sup>3</sup> = 4...
<p>Different ELISA setups (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.011...
<p>The response of the ELISA Systems sandwich assays to a commercial lager, (Tanner this journal, th...
<p>The scheme introduces the six sandwich ELISA protocols that were compared in the study.</p
<p>The detection of NS1 in culture supernatants of WNV-infected cells by different MAb pairs. The cu...
<p>10<sup>6</sup> yeast displaying the indicated antibodies were induced, washed and resuspended in ...
<p>(A) A group of typical signal-time traces at different concentrations of <i>E. coli</i> O157:H7 d...
<p>The response of ELISA Systems sandwich assay containing total Sloop hordein (500 ppb) and either ...
Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens...
Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens...
<p>A series of primary antibody dilutions (1:500, 1:600, 1:700, 1:800, 1:900 and 1:1000) in PBS were...