p17 inhibits CDK1 and vimentin phosphorylation at Ser 56 and Ser 82 by p53/Plk1pathways.

<p>The levels of Tpr, p-p53, Plk1, p-Plk1 and cell cycle regulatory proteins were examined in ARV-infected (left panel) and p17-transfected (right panel) vero cells. ARV infection and p17 transfection increased the phosphorylated form of CDK1 and reduced the phosphorylated forms of vimentin at Ser 56 and Ser 82. Vero cells were mock-infected, ARV-infected with a MOI of 5, and p17-transfected for 24 h. Cell lysates were collected and phosphorylation and protein levels were analyzed by Western blot assays with the respective antibodies. The protein levels were normalized to that for β-actin. The fold activation and inactivation indicated below each lane were normalized against the values for mock-infection or mock-transfection. The levels of the indicated proteins in the mock controls were considered 1-fold. The experiments were repeated three times, and representative blots are shown. The uncropped blots with molecular weights are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0162356#pone.0162356.s012" target="_blank">S12 Fig</a>.