<p>Immunoblotting showed that the phosphorylated Smad3 (p-Smad3) level and the ratio of p-Smad3 to Smad3 were significantly decreased in the MDCK cells treated with TGF-β1 and AAT compared to that in MDCK cells treated with TGF-β1 alone (A to D). The level of phosphorylated Smad2 (p-Smad2) and the ratio of p-Smad2 to Smad2 were significantly increased in MDCK cells after treatment with TGF-β1 and showed tendency to decrease in MDCK cells treated with TGF-β1 and AAT (E to H).</p
The effect of a 30 minute treatment with 10ng/ml TGF-β1 on the expression of phosphorylated Smad2 (p...
<p>The iPSCs were cultured in iPSC medium, and treated with TGFβ inhibitor SB431542 (10μM) for 3 day...
NRK52E cells were treated with TGF-β1 (10 ng/ml) with or without HL156A (30 μM) for 24 hours. (A) Im...
<p>A, The endogenous Smad4 protein levels were analyzed in SBOT3.1 and MPSC1 cells using a western b...
<p>TGF-β1 exposure increased the expression of α-SMA (A and B) and decreased the expression of E-cad...
<p>(A) Huh7-shRNA-Smad3 cells (Clone 3) were transfected with increasing amounts of Myc-Smad3 expres...
<p>(<b>A</b>) MCF-7 cells were pre-treated with ADC (5–20 μM) for 2 h prior to stimulation with TGF-...
<p>(A) and (B) SVOG cells were transfected with 50 nM control siRNA (si-Ctrl) or Smad2 siRNA (si-Sma...
<p>Cells were cultured with or without 5 ng/ml TGF-β for 24 h. Changes in Smad2, Smad3 and Smad4 lev...
<p>(A) Effects of KY-05009 on TGF-β1-activated Smad signaling. Serum-deprived A549 cells were treate...
Although Smad2 and Smad3, critical transcriptional mediators of transforming growth factor-beta (TGF...
<p>(A, B) Western blotting was performed to detect the protein levels of p-Smad3 and Smad3, and beta...
<p>(A) RT-PCR analyses showed that TGF-β1 treatment led to downregulation of Smad3 mRNA expression, ...
<p>(A) RPE cells were cultured in the absence or presence of TGFβ2 for 15min, 30 min and 60 min, the...
<p>(A) Smad3 and Smad2 activation, assessed by pSmad3 or pSmad2 immunoblotting, in NMuMG cells, tran...
The effect of a 30 minute treatment with 10ng/ml TGF-β1 on the expression of phosphorylated Smad2 (p...
<p>The iPSCs were cultured in iPSC medium, and treated with TGFβ inhibitor SB431542 (10μM) for 3 day...
NRK52E cells were treated with TGF-β1 (10 ng/ml) with or without HL156A (30 μM) for 24 hours. (A) Im...
<p>A, The endogenous Smad4 protein levels were analyzed in SBOT3.1 and MPSC1 cells using a western b...
<p>TGF-β1 exposure increased the expression of α-SMA (A and B) and decreased the expression of E-cad...
<p>(A) Huh7-shRNA-Smad3 cells (Clone 3) were transfected with increasing amounts of Myc-Smad3 expres...
<p>(<b>A</b>) MCF-7 cells were pre-treated with ADC (5–20 μM) for 2 h prior to stimulation with TGF-...
<p>(A) and (B) SVOG cells were transfected with 50 nM control siRNA (si-Ctrl) or Smad2 siRNA (si-Sma...
<p>Cells were cultured with or without 5 ng/ml TGF-β for 24 h. Changes in Smad2, Smad3 and Smad4 lev...
<p>(A) Effects of KY-05009 on TGF-β1-activated Smad signaling. Serum-deprived A549 cells were treate...
Although Smad2 and Smad3, critical transcriptional mediators of transforming growth factor-beta (TGF...
<p>(A, B) Western blotting was performed to detect the protein levels of p-Smad3 and Smad3, and beta...
<p>(A) RT-PCR analyses showed that TGF-β1 treatment led to downregulation of Smad3 mRNA expression, ...
<p>(A) RPE cells were cultured in the absence or presence of TGFβ2 for 15min, 30 min and 60 min, the...
<p>(A) Smad3 and Smad2 activation, assessed by pSmad3 or pSmad2 immunoblotting, in NMuMG cells, tran...
The effect of a 30 minute treatment with 10ng/ml TGF-β1 on the expression of phosphorylated Smad2 (p...
<p>The iPSCs were cultured in iPSC medium, and treated with TGFβ inhibitor SB431542 (10μM) for 3 day...
NRK52E cells were treated with TGF-β1 (10 ng/ml) with or without HL156A (30 μM) for 24 hours. (A) Im...