Familiarity to a Feed Additive Modulates Its Effects on Brain Responses in Reward and Memory Regions in the Pig Model - Fig 5

<p><b>Feed intake (g) during the (A) 1-h 2-choice feeding tests and the (B) 6-h 2-choice feeding tests in the naive (NAI) and familiarized (FAM) groups.</b> All animals were fed a first stage pre-transition diet from weaning to d 15, with (FA) or without (C) additive. On d 16, a feed transition was performed and the pre-transition diet was replaced by a post-transition diet of different composition, with (FA) or without (C) the same additive. During the 2-choice tests performed on d 16 and d 23, the piglets had the choice between the post-transition diet without additive and the same diet with the additive. Data are represented with means ± SEMs. Percentages in bars indicate diet intake relative to total intake. <i>t</i> indicates trend for the FAM group to consume more FA feed than the NAI group during the 6-h tests on d 16 (p = 0.14) and d 23 (p = 0.10). The asterisk indicates a moderate preference for the feed, <i>i</i>.<i>e</i>. a percentage of feed intake from 60 to 80% of total feed intake, according to the scale of Goatcher and Church described in Kennedy and Baldwin [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0162660#pone.0162660.ref015" target="_blank">15</a>].