Inhibition of mitochondrial respiration by compound 7.

<p>(A) Effect of compound 7 on mitochondrial respiration. OCR was measured using XF24 extracellular flux analyzer by adding oligomycin (1 μM), FCCP (0.5 μM), and rotenone (1 μM)/antimycin A (1 μM) to compound 7-treated HCT116 cells. (B) Effect of compound 7 on intracellular oxygen tension. HCT116 cells were treated with compound 7 for 6 h under hypoxia condition. The intracellular oxygen tension was detected by hypoxia-sensitive probe MAR (0.5 μM). The scale bar indicates 200 μm. The fluorescence intensity of MAR probe was shown by vertical bars. Statistical significance: **<i>P</i> < 0.01, compared with hypoxia control.