Characterization of Pichia-expressed recombinant stLHβ, stLHβα, stFSHβ and stFSHβα by Western-blot analysis and deglycosilation.

<p>Supernatants of transformed Pichia cultures were separated by 15% semi-native PAGE and immunoreacted with antibodies raised against anti-His (A) recombinant stLHβ (B) or recombinant stFSHβ (C). For deglycosylation analysis, denatured and reduced proteins were incubated with (lanes 2, 4, 6, 8 and 10) or without (lanes 1, 3, 5, 7 and 9) N-glycosidase F. stLHβ: lanes 1,2; stLHβα: lanes 3,4; stFSHβ: lanes 5,6; stFSHβα: lanes 7,8; sturgeon pituitary extract (diluted 1:100): lanes 9,10; PM-protein marker; HM-His-tag protein marker.