PDGFRα⁺ OPCs become YFP-labelled throughout the nervous system of adult <i>Pdgfrα-CreER</i>^<i>T2</i>::<i>Rosa26-YFP</i> transgenic mice.

<p>Cryosections from P57+7 <i>Pdgfrα-CreER</i>^<i>T2</i>::<i>Rosa26-YFP</i> transgenic mice were immunostained to detect PDGFRα (red), YFP (green) and Hoescht 33342 (HST, blue). Confocal image (single z plane) of the motor cortex at low (a) and high (b) magnification. Confocal image (single z plane) of the corpus callosum (cc) at low (c) and high (d, e) magnification. Confocal image (single z plane) of a transverse spinal cord section at low (f) and high (g) magnification. Confocal image (single z plane) of the retina (h). Confocal image (single z plane) of a transverse sciatic nerve section at low (i) and high (j, k) magnification. Cryosection from P57+7 <i>Pdgfrα-CreER</i>^<i>T2</i>::<i>Rosa26-YFP</i> transgenic mouse immunostained to detect S100β (red), YFP (green) and HST (blue) (l). m) The number of PDGFRα⁺ YFP⁺, PDGFRα⁺ YFP-negative and PDGFRα-negative YFP⁺ cells quantified from confocal images (single z plane) of nervous system tissue, expressed as the number of cells per mm². n) The proportion of PDGFRα⁺ cells that become YFP-labelled (PDGFRα⁺ YFP⁺ cells / total PDGFRα⁺ cells x 100) quantified from confocal images (single z plane) of nervous system tissue. Error bars represent mean ± std dev from n = 3 mice. ** P<0.001, ANOVA. PDGFRα⁺ YFP⁺ cells (and S100β⁺ YFP⁺ cells) are denoted by arrowheads. PDGFRα-negative YFP⁺ cells are denoted by arrows. Scale bars represent 50μm.