Effect of stable <i>TFF3</i> overexpression on RB cell colony formation capacity.

<p>(A) Quantification of soft agarose assays showing a significant lower capacity of TFF3 overexpressing WERI-Rb1 cells to form colonies. (B) Photographs taken from soft agarose colonies at the day of seeding (day 0), 1, 2 and 3 weeks in culture revealing that <i>TFF3</i> overexpressing WERI-Rb1 cells (TFF3) show the tendency to form smaller colonies than control cells (ctr). Scale bar: 50 μm; applies to all photographs. Values are means from 3 independent experiments ± SEM. **P < 0.01 compared to the control group calculated by Student`s <i>t</i>-test.