QSYQ time-dependently inhibited the fibrotic activity mediated by TGF-β1 in NRK 52E cells.

<p>NRK 52E cells were pre-incubated with TGF-β1 (10 ng/ml) and treated with QSYQ (20 μg/ml) 0, 12, and 24h after TGF-β1 incubation, respectively. Cells were harvested 36 h after TGF-β1 stimulation for real-time PCR and 48 h after TGF-β1 stimulation for Western blot analysis, respectively. (A) Real-time PCR analyses of α-SMA, collagen I, and fibronectin. (B) Western blot analyses of α-SMA, collagen I, and fibronectin. *<i>p<</i>10⁻⁸ vs TGF-β1 and QSYQ untreated cells. ANOVA, <i>p<</i>10⁻⁸ for QSYQ-treated cells in A and B. Data are expressed as the mean ± SD of three independent experiments.