Relative bioavailability of CSE soluble components after exposure to different cell counts.

<p>(<b>A</b>) In this transfer assay, different cell numbers (0, 25,000; 50,000 cells/well) were exposed to one of two volumes of CSE (0.5 mL or 1 mL) for 60 minutes. After this short exposure, 400 uL of the resulting CSE solution was removed from the wells, and added to new undamaged cells (15,000 cells/well). These cells were exposed to the CSE solution for an additional 24 hours, before (<b>B</b>) analysis by MTT, and calculation of relative bioavailability compared to 100% bioavailability defined as equivalent to the cellular response to the amount of CSE available after transfer from wells with a high volume of CSE solution, and no cells present. Error bars represent the standard error of the mean. n.s. represents a lack of significance compared to other designated experimental conditions, p > 0.05.