Effects of cell count on cytotoxicity biomarkers after CSE exposure, using the LDH release assay.

<p>(<b>A</b>) NIH 3T3 mouse fibroblasts were plated at 10,000, 15,000, or 20,000 cells/well in a 48 well plate, allowed to grow for 24 hours, and and exposed to CSE for 24 hours using 400 uL of 0–7% CSE. An MTT viability assay was then used to assess relative cellular damage. Values are reported as % viability compared to cells not exposed to CSE <b>(B)</b> Cells were plated and exposed to CSE as stated above, then a lactate dehydrogenase assay was performed to assess the amount of lactate dehydrogenase released. Values are reported as % cytotoxicity compared to cells exposed to 20% CSE (which was considered maximum LDH release), with error bars representing the standard error of the mean. * Compared to other cell counts exposed to the same percent CSE, p < 0.05.