Alignment of amino acid positions among peptides predicted from 45 alleles found for the <i>gp63</i> gene fragment studied.

<p>The 45 translated alleles were detected among 41 different isolates of <i>L</i>. <i>(V</i>.<i>) braziliensis</i> from ATL patients of Corte de Pedra. Alleles 1 to 28 derived from 35 parasite isolates obtained from ATL patients between 1992 and 2001. Alleles 29 to 45 derived from 6 parasite isolates obtained from ATL patients between 2008 and 2011. Alignment was generated by MEGA 5.0 software. Numbers boxed in top three rows correspond to amino acid positions in the <i>gp63</i> gene used a reference (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163284#sec002" target="_blank">methods</a>). Top sequence in bold corresponds to the amino acids found for each displayed position in the reference <i>gp63</i> sequence. Rows numbered 1 to 45 at left correspond to the 45 alleles of <i>gp63</i> detected in Corte de Pedra. Dots indicate same amino acids as in reference sequence, while letters indicate the substituting amino acids in the study fragments. Regular letters indicate hydrophilic, italicized letters indicate intermediary, and bold letters indicate hydrophobic amino acids. Shaded positions correspond to the segments PAVGVINIPA, SRYD, HEVAH and KAREQYGC referred to in text.