Multiple bat species detected in each of eight mines in the U.S. Southwest from next-generation amplicon sequencing of pooled guano.

<p>A single DNA extraction was performed on a pooled sample of roughly 200 fecal pellets that were collected from each mine, and the Species from Feces primer pair SFF_145f/SFF_351r was applied with next generation amplicon sequencing on an Illumina MiSeq. *Mine sample NM_1 also contained small proportions of <i>Corynorhinus townsendii</i> and <i>Myotis thysanodes</i>/<i>evotis</i>.