GDE5dC471 overexpression in skeletal muscle increases neuromuscular junctions-related mRNA expression.

<p><i>A</i>, Total RNA from gastrocnemius muscle of GDE5dC471 mice (Tg) and age-matched control mice (Wild) (4-week-old, n = 4; 8-week-old, n = 3; and 1-year-old, n = 5) was subjected to quantitative PCR to examine mRNA expression level of genes related to neuromuscular junctions. Data represent mean ± SE. *<i>p</i><0.05, **<i>p</i><0.01. <i>B</i>, Representative photographs of fluorescent α-bungarotoxin staining in transverse sections of gastrocnemius muscle, Left; 1-year-old of GDE5dC471 mice (Tg), and right; age-matched control mice (Wild). No morphological alteration of nicotinic acetylcholine receptor (nAchR) was observed between GDE5dC471 and control mice.