Impaired central insulin signaling after 2 weeks of diet.

<p>(A) Representative immunoblots and (B) densitometry of the ratio of the serine phosphorylation of IRS₁ at the 636 residue (pIRS₁^S636) to the tyrosine phosphorylation of IRS₁ at the 1222 residue (pIRS₁^Y1222), IRS₁, and total insulin receptor (InsR) in CTRL and HF mice after 2 weeks of diet. *P<0.05 compared with CTRL mice. (C) Representative immunoblots and (D) densitometry of pAKT following <i>ex vivo</i> insulin stimulation. #P<0.0001 compared with non-insulin treated CTRL mice. All data represent mean ± SEM. n = 6 per group.