Effect of miltefosine on AMPK signal pathway in cultured primary mouse hepatocytes.

<p>Primary hepatocytes were cultured in serum-free medium overnight and treated with miltefosine for the indicated times. (A) Representative western blots of AMPKα, ACC, IRS2, and SREBP1C. (B) Effect of miltefosine on CPT1A and LKB1 expression. Cells were treated with miltefosine for 24 hours and cell lysates were quantified by western blot. (C) Nuclear and cytosolic levels of LKB1 were determined by western blot. (D) High glucose on AMPK activity in liver cells. (E) High glucose on AMPK signal pathway in liver cells. (F) High glucose on expression levels of CPT1A and LKB1 in liver cells. Data are expressed as the mean ± SEM. n = 5. * P < 0.05, *** P < 0.001.