Expression of <i>Axin2</i> in chicken limb development.

<p>(A.1, wing bud, w) HH 17, strong expression of <i>Axin2</i> in the limb, predominantly in the dorsal most part (black arrow). (A.2) The leg bud (l) shows less staining, a patch in the ventro-proximal tissue expresses <i>Axin2</i> (black arrow). (B.1, leg bud) HH 18, transcripts in the dorsal mesenchyme and in the distal ectoderm. (C.1) HH 19, expression in the dorsal and most proximal mesenchyme of the wing bud. (HH 19, C.1; HH 20, D.1 black arrow; D.2) <i>Axin2</i> expression <i>aer</i>. (C.1) HH 19 and (D.1) HH 20, <i>Axin2</i> in dorsal subectodermal mesenchyme, in ectoderm and in the <i>aer</i>. (E.1, E.2) HH 23; (F.1) HH 25: Transcripts in the ectoderm and <i>aer</i> of the limb buds. (E.2–I.3) HH 23 to HH 28: the ectoderm expresses high levels of <i>Axin2</i>. (G.1) HH 26, black arrow; (I.3) HH 28, black arrow: the margins of the developing <i>bo</i> show transcripts of <i>Axin2</i>. aer-apical ectodermal ridge, bo-bones.