Lung cell characterization following infection with H9N2/G1 and the PB2 mutants.

<p>BALB/c mice were infected intranansally with 1.5x105 PFU of H9N2/G1 or the PB2 mutants. Cell sub-populations (A) T cells (B) Neutrophils (C) Macrophages (D) Dendritic cells (E) NK cells were collected from the lungs and were gated with antibodies at various times post-inoculation. The data shown represents the mean number of cells and standard deviations from four mice per time point, * p<0.05 compared to the H9N2/G1wt. (F-J) The percentage of NP positive cells from the subpoplutions. Gating: T cells(CD3+/CD11c-/CD11b-/Ly6G/C-), Neutrophils(CD11b+/CD11c-/CD3-/Ly6G/C+),Macrophages(CD11b+/CD11c-/CD3-/Ly6G/C-), Dendritic cells(CD11c+/CD11b-/Ly6G/C-/CD3-), NK cells(Dx-5+/CD3-). The data is the representative results from one of two independent experiments.