The cerebellum of NBRT treated mice reveals early astrocytosis and microgliosis.

<p>In the cerebellum of the mice administered NBRT sacrificed at day 3, increased numbers of GFAP⁺ astrocytes (C, black arrow) were identified as compared to CONT mice (A, black arrow). No significant differences in astrocytes were noted when MTX mice (E, black arrow) were compared to NBRT mice. Increased numbers of Iba1⁺ microglia (D, white arrows) were identified as compared to CONT mice (B, gray arrows). No significant differences were noted when MTX mice (F, white arrows) were compared to the NBRT mice (D). In contrast to the small, minimally ramified microglia in the CONT mice (B, gray arrows), the microglia in the NBRT and MTX treated mice demonstrated features consistent with activation (D and F, white arrows).