TrkC.T1 expression is up-regulated whereas miR-128 expression is down-regulated in the spinal cord of ALS G93A mice and in human SALS.

<p><b>(A)</b>The mRNA levels of TrkC-FL and TrkC.T1 were assessed by quantitative PCR with SYBR Green (Quanta), normalized to GAPDH or β-tubulin III, for WT mice or ALS mice. Data are expressed as the mean + SEM (n = 8 spinal cord samples each group<b>(B)</b> The levels of miR-128 (regulator of TrkC.T1) and miR-151-3p (regulator of TrkC-FL) were measured by SYBR Green qRT-PCR (Quanta) and results were normalized to the reference RNU6 (small nuclear RNA). Data are expressed as the mean + SEM. <b>(C)</b> Combined fluorescent in situ mRNA hybridization (red, for TrkC.T1 mRNA) with immunofluorecence (green, GFAP marker). 40x magnification of 20 μm-thick sections of the lumbar spinal cord of G93A ALS mice. The probe specific for TrkC.T1 encompasses exons 13b & 14b as well as some of the 3'UTR. <b>(D)</b> Co-localization of TrkC.T1 protein (using anti-TrkC.T1 antibody 750) and GFAP in 12 μm thick sections from G93A ALS mice (top panels) or wild type mice (bottom panels). Pictures were taken using a Leica confocal microscope with 63x magnification. <b>(E)</b> The mRNA levels of TrkC-FL and TrkC.T1 were assessed by quantitative PCR with SYBR Green (Quanta), normalized to β-tubulin III, for human control (n = 3) or SALS (n = 4). Data are expressed as the mean + SEM. <b>(F)</b> From the same human samples the levels of miR-128 (regulator of TrkC.T1) and miR-151-3p (regulator of TrkC-FL) were measured by SYBR Green qRT-PCR (Quanta) and results were normalized to the reference RNU6 (small nuclear RNA). Data are expressed as the mean + SEM. <b>(A–F)</b> t-test was applied for statistical analysis. * p<0.05, ** p< 0.01 versus control.