The LplA1 ligase is necessary for salvage of lipoic acid during in vitro growth.

<p>(A, C, and E) Growth curves of the indicated strains in (A) RPMI, (C) RPMI + 25 nM lipoic acid (LA) or (E) RPMI + 250 μM octanoic acid (OA). (B, D, and F) Whole cell lysates of <i>S</i>. <i>aureus</i> collected after 9 hours of growth in (B) RPMI + BCFA (2-methyl butyric acid, isovaleric acid, isobutyric acid, and sodium acetate), (D) RPMI + BCFA + 5 μM lipoic acid (LA), or (F) RPMI + BCFA + 175 μM octanoic acid (OA) followed by immunoblotting for lipoic acid-containing proteins. In all growth curves, the mean +/- standard deviation of triplicate measures is shown. In any case where an error bar is not visible, the standard deviation was smaller than the size of the symbol used at that data point.