DLS analysis of lens proteins upon modification with Ac₂O and HCTL.

<p>Lens proteins were assessed for their hydrodynamic size distribution, upon modification with Ac₂O and HCTL, by DLS. Prior to the measurements, the protein samples were diluted to 3 mg mL ^-1 in 100 mM phosphate buffer, pH 7.4. The size distributions were reported to their relative volumes. The insets indicate their relative scattering intensity. A, B, C and D, respectively indicate native proteins, acetylated proteins, HCTL-modified proteins and double modified proteins.