<p>①, ②, ③ were three pair primers designed for each large indel, and yield three amplicons AP1, AP2, AP3 respectively. BC was the inserted or deleted sequence.</p
<p>Features of primer pairs (ΔTm) are counted once, features of single primers (ΔG) were counted sep...
<p>All sequences are written in the 5′ to 3′ direction. The various modifications are indicated. Low...
<p>Primer sequences used for 454 sequencing are found in <a href="http://www.plosone.org/article/inf...
<p>*The FIP primer consisted of F2 and the complementary strand of F1 (F1c).</p><p>**The BIP primer ...
<p>Details of DNA sequence alignments used for primer design, including the numbers of taxa, alignme...
(A) MIEN1 deletion schematic. Blue arrows represent location of primers for deletion PCR reaction. B...
<p>Sequence of primers used in real-time PCR, amplicon sizes and annealing temperatures.</p
<p>V6 rRNA region were amplified using the two primers with barcode tags and Illumina adaptors attac...
<p>Forward and reverse primers designed based on the DNA-sequence of contigs.</p
<p>The final 496 bp contig was assembled from five amplicons (primer pairs 1+2; 3+4; 5+9; 3+6; 7+8)....
Amplicon sequences generated (and their corresponding length) by each of the primer pairs used in th...
<p>The arrows represent PCR primers. The sequences of linkers and PCR primers are listed in <a href=...
<p>The same primers were used for sequencing amplicons, except that M13F was used to sequence strand...
1<p>An s in a primer's name indicates that it was used for sequencing only. Primers marked with n we...
The direction of the arrows indicated the direction of primers extension and amplification. A, Prime...
<p>Features of primer pairs (ΔTm) are counted once, features of single primers (ΔG) were counted sep...
<p>All sequences are written in the 5′ to 3′ direction. The various modifications are indicated. Low...
<p>Primer sequences used for 454 sequencing are found in <a href="http://www.plosone.org/article/inf...
<p>*The FIP primer consisted of F2 and the complementary strand of F1 (F1c).</p><p>**The BIP primer ...
<p>Details of DNA sequence alignments used for primer design, including the numbers of taxa, alignme...
(A) MIEN1 deletion schematic. Blue arrows represent location of primers for deletion PCR reaction. B...
<p>Sequence of primers used in real-time PCR, amplicon sizes and annealing temperatures.</p
<p>V6 rRNA region were amplified using the two primers with barcode tags and Illumina adaptors attac...
<p>Forward and reverse primers designed based on the DNA-sequence of contigs.</p
<p>The final 496 bp contig was assembled from five amplicons (primer pairs 1+2; 3+4; 5+9; 3+6; 7+8)....
Amplicon sequences generated (and their corresponding length) by each of the primer pairs used in th...
<p>The arrows represent PCR primers. The sequences of linkers and PCR primers are listed in <a href=...
<p>The same primers were used for sequencing amplicons, except that M13F was used to sequence strand...
1<p>An s in a primer's name indicates that it was used for sequencing only. Primers marked with n we...
The direction of the arrows indicated the direction of primers extension and amplification. A, Prime...
<p>Features of primer pairs (ΔTm) are counted once, features of single primers (ΔG) were counted sep...
<p>All sequences are written in the 5′ to 3′ direction. The various modifications are indicated. Low...
<p>Primer sequences used for 454 sequencing are found in <a href="http://www.plosone.org/article/inf...