LC3β immunofluorescence staining of human samples to identify autophagic induction at various histological stages of carcinogenesis.

<p>Panels A-C are at 40x magnfication while Panels A-2 to C-2 are acquired at 63x magnification. Panel A contains a representative normal human anal specimen with evidence of punctate LC3β. Panel B demonstrates continued LC3β punctate formation with low-grade squamous intraepithelial lesion (LSIL) which equates to low-grade dysplasia in the mouse samples. Panel C depicts a human anal sample with high-grade squamous intraepithelial lesion (HSIL) which equates to high-grade dysplasia in the mouse samples, and continues to show evidence of punctate LC3β. These images demonstrate, as in the K14E6/E7 mouse samples, that autophagic induction is intact throughout anal carcinogenesis.