Tuberculosis and sarcoidosis have similar differentially regulated genes.

<p><b>(A)</b> Differentially expressed genes identified from new dataset for TB and sarcoidosis groups compared to healthy controls (transcripts filtered which were not significantly detected from background in at least 10% of samples, low expressed transcripts filtered (less than 2 fold change from median in 10% of samples), followed by statistical testing (independent t-test with Benjamini Hochberg multiple testing correction (<i>q-value</i> <0.05) between groups of interest). Transcripts were matched to Entrez gene identifiers and duplicates and non-matched transcripts filtered. Venn diagram showing the overlap of DEGs between these two diseases and the most significant 15 gene list from the meta-analysis. <b>(B)</b> Heatmap of normalised expression using the Bloom et al 144 transcript list are able to broadly differentiate pulmonary TB patients from sarcoidosis patients. Clustering (Pearson’s uncentred (Cosine) with averaged linkage) on transcripts (rows) and individual patient blood samples (columns). <b>(C)</b> The same 144 transcript list (16) is unable to differentiate between mediastinal TB patients and sarcoidosis patients by clustering (as before) <b>(D)</b> Molecular scores calculated for transcripts from 380 gene meta-signature, MDTH and transcripts representing the interferon modules (healthy controls acting as control group). Z scores calculated (with healthy controls used as reference group for calculating mean and SD) and then TB patients and sarcoidosis patients ranked according to Z score of the 380 gene meta-signature. For all three outcomes the mean of pulmonary TB patients was significantly higher than both the extra-pulmonary TB and sarcoidosis patients (p<0.05). Differences among means were tested with a generalized linear model assuming a normal distribution and a Bonferroni multiple testing correction. No symptoms indicates absence of any of the five symptoms listed previously.