Validation of host nucleolin and viral nucleoprotein interaction in cells infected with influenza A viral seasonal H1N1 and H3N2 strains.

<p>A549 cells grown on cover slips were infected with seasonal strains as described earlier. At every 2hrs intervals up to 10hrs cells were fixed and subjected to double IFA staining and analyzed by confocal microscopy. Co-localization of viral NP and host nucleolin at 4–6hrs in cells infected with [a] H1N1 [b] H3N2 strain (Contrary to H1N1 staining, secondary antibody with TRITC was used to stain viral NP and FITC to stain viral NP in cells infected with H3N2).