Reduction of NAD results in large metabolic changes.

<p>A) Levels of 97 different metabolites were assessed in each cell line as described in the methods section at 24, 48 or 72 hours after treatment with 0.4 μM GNE-617 (n = 5, average of each metabolite is shown). Shown is the log2-fold change for the level of each metabolite relative to its level in untreated cells. B) Changes in different categories of metabolites at 72 hours in each cell line, as determined in panel A.