Expression of HA antigens in rVHSV-HA or rIHNV-HA infected cells.

<p>The expression of HA antigens was assessed by indirect immunofluorescence assays on EPC cells. The cells were infected with either rVHSV-HA or rIHNV-HA and incubated at 14°C. (A) At 24 h post-infection, cells were fixed and permeabilized with alcohol/acetone and HA expression was detected using a monoclonal antibody against HA1 (H36-26). (B) At 48 h post-infection, membrane expression of HA antigen was visualized on live cells in PBS using H36-26 monoclonal antibody (magnification x40).