RT-PCR analysis of bovine <i>Lhx8</i> and <i>Lhx8-v1</i> mRNA expression.

<p><b>A</b>: Expression of <i>Lhx8</i> and <i>Lhx8-v1</i> mRNA in bovine tissues. Tissues tested include spleen, stomach, brain, muscle, kidney, liver, heart, intestine, adult ovary, adult testis, fetal testis and fetal ovary. <b>B</b>: Expression of <i>Lhx8</i> and <i>Lhx8-v1</i> mRNA in bovine fetal ovaries from different gestation stages. Fetal ovaries from 90, 95, 100, 150, 160, 200, 210, 230 and 250 day fetuses were analyzed. The ages of fetuses were estimated based on crown-rump length. <b>C</b>: Expression of <i>Lhx8</i> and <i>Lhx8-v1</i> mRNA in oocytes and early embryos. Oocytes and embryos samples used in the analysis include GV- and MII-stage oocytes and 2-cell, 4-cell, 8-cell, 16-cell, and morula- and blastocyst-stage embryos. Bovine <i>RPL19</i> gene was used as a control for RNA quality.