Additional file 3: of Genome-wide association study for hereditary ataxia in the Parson Russell Terrier and DNA-testing for ataxia-associated mutations in the Parson and Jack Russell Terrier

Primer pair sequences, restriction enzymes, annealing temperature (AT) and amplicon size used for genotyping the mutations CAPN1:c.344G > A and KCNJ10:c.627C > G by PCR-RFLP are given. Primer pair sequences, annealing temperature (AT) and amplicon size of PCR for genotyping the mutation KCNJ10:g.22141027insC validated on a LI-COR 4300 DNA Analyzer as well as primer pair sequences and product-sizes for Sanger sequencing PCR-amplicons of exon 2 and 3 of KCNJ10 are shown. (DOC 57 kb