Endogenous Reference Genes for Gene Expression Studies on Bicuspid Aortic Valve Associated Aortopathy in Humans - Fig 1

<p><b>(a) Expression stability of housekeeping (HK) genes.</b> The graph shows the average expression stability value (M) for each HK gene ranked according to increasing stability with the most stable genes (lowest M values) on the right (geNorm 3.4). <b>(b) The number of genes required for normalisation.</b> The graph shows the levels of variation in average HK gene stability with the addition of further housekeeping genes to the equation. The two most stable genes are included first with the inclusion of a 3rd, 4th, 5th gene etc. moving to the right (geNorm 3.4). The graph indicates that the two most stable genes created a normalization factor which is not significantly altered by the addition of more genes as they all have a V score below 0.15.