High content microscopy identifies compounds that increase the F508del CFTR pool.

<p>(A) Cartoon depicts subcellular regions of interest (ROI) for image analysis in a high throughput screen of small molecules. With this method, compounds can be identified that influence either plasma membrane or total cellular expression of F508del CFTR, with representative immunofluorescence image also shown. (B) Fluorescence intensity distribution within key regions of interest in vehicle treated samples within a high content screen of 2,000 compounds from the NIH Roadmap Molecular Libraries Small Molecule Repository. (C) PubChem identifiers for a subset of small molecules that augment total cellular and/or surface expression of F508del CFTR. Each value shown is >3 standard deviations above mean fluorescence intensity for all compounds tested.