Rv treatment downregulates apoptotic pathway.

<p>Adult mosquitoes were reared until six days old under each dietary condition. The midguts of female mosquitoes were dissected and homogenized in TRIzol, and total RNA was extracted. These samples were used to perform qPCR for the genes: (<b>A</b>) 16S, (<b>B</b>) ARGONAUTE 2, (<b>C</b>) CASPASE 16, (<b>D</b>) AeDRONC, (<b>E</b>) AeIAP1 and (<b>F</b>) ATG8. Data show means and standard error of at least three independent experiments. Ctrl—0.05% ethanol plus 10% sucrose; Rv—100 μM Rv in 0.05% ethanol plus 10% sucrose; AbMix– 10% sucrose, 10 U/mL penicillin, 10 U/mL streptomycin and 15 U/mL gentamicin. *—p<0.05; **—p<0.01; ***—p<0.001; ****—p<0.0001, as calculated by Student’s t-test (F as calculated by one-way ANOVA with Tukey post test). (Error bars, s.e.m., n = 3 experiments).