Mass spectrometric-based comparative proteomic analysis of active vs. non-active fractions of McES.

<p>(A) Heat map showing the mean relative abundance of the indicated proteins identified in all McES fractions investigated. (B) Bivariant plot of NSAF values of proteins from active and non-active McES fractions. McES fractions after gel filtration chromatography were separated into active vs. non active depending on their ability to impair LPS-driven BMDC activation. 3–4 fractions from the most active and non-active fractions were lyophilized and analysed by LC-MS/MS. Proteins exclusively present in active fractions are displayed in grey boxes in a descending order of NSAF values. For the active or non-active group of fractions, the computed NSAF value of a given protein is the mean of the individual NSAF values of this protein in the 3–4 different fractions analyzed.