<i>In-Vivo</i> Detection and Tracking of T Cells in Various Organs in a Melanoma Tumor Model by ¹⁹F-Fluorine MRS/MRI - Fig 4

<p>(<b>A</b>) PCF labelling of total splenocytes (SP), anti-CD3/anti-CD28 activated (T_act) and Ova-peptide activated (T_OVA-act) splenocytes from OT-1 cells. (<b>B</b>) Phenotype of CD3⁺ T cells: CD3⁺CD8⁺ T cells were analyzed by flow cytometry for CD44, CD62L, CD127 and PD-1 expression. By this flow-cytometric analysis, the following phenotypes could be identified in the CD3⁺CD8⁺ T-cell population: CD62L⁺CD44^- (naive T cells), CD62L⁺CD44⁺ (memory T cells) and CD62L^-CD44⁺ (effector T cells). n = 3 for each group of stimulation. Data are representative of 3 independent experiments.