Structures and dose-response relationships for three inhibitors of PPIP5Ks.

<p>Structures for (A) UNC10112646 (B) UNC10225354 and (C) UNC10225498 (D) Dose-response curves for the inhibition of PPIP5K by UNC10225354 (IC₅₀ = 5.24 ± 0.18 μM), UNC10225498 (IC₅₀ = 2.14 ± 0.07 μM), and UNC10112646 (IC₅₀ = 6.96 ± 0.03 μM). (E) Counterscreen results for the three inhibitors performed in the absence of PPIP5K and 5-InsP₇ show that these inhibitors do not interfere with the detection reagents and assay signal. In these experiments, 100% activity is equivalent to consumption of 19.5 ± 0.8% of the ATP.