Histological analysis and angiogenic growth factors expression in wound tissue.

<p><b>(a)</b> Samples were isolated from diabetic rats (D) and diabetic rats treated with linoleic acid (DLA) at the 1^st, 3^rd, 7^th and 14^th days after wounding. <b>(b)</b> Representative new vessel formation in wound tissue from the D and DLA groups. Samples were collected on the 7^th day after wounding. <b>(c)</b> Vessels quantification. Results are presented as mean ± SD. D (4 rats) and DLA (5 rats). (*) Indicates significant difference between D and DLA (p = 0.0001). <b>(d)</b> TGF-β mRNA expression and VEGF concentration in wound tissues from diabetic rats (D) and diabetic rats treated with linoleic acid (DLA). Results are presented as mean ± SD. D (9 rats) and DLA (4 rats). (*) Indicates significant difference between D and DLA (VEGF–p<0.01). <b>(e)</b> eNOS and ANGPT-2 mRNA expression in wound tissues from diabetic rats (D) and diabetic rats treated with linoleic acid (DLA). Results are presented as mean ± SD. D (9 rats) and DLA (4 rats). (*) Indicates significant difference between D and DLA (ANGPT-2 –p = 0.01). V: vessel. DE: derm. Objective 10X.