DR reduces adult size and early fecundity.

<p>A,B) Representative adult worms after 96 hr in culture for WT and <i>eat-2(ad465)</i> are shown. C,D) Representative adult worms cultured in AL or DR for 96 hr (starting from L1 arrest) are shown. Scale bars in A-D are 200 μm. E) Adult length (96 hr after L1 arrest) is plotted for WT and <i>eat-2(ad465)</i>. F) Adult length (96 hr after L1 arrest) is plotted for AL and DR. Mean and SEM are plotted in E and F (*p<0.05, paired t-test, n = 3 and 4, respectively). G,H) Representative DAPI-stained worms cultured in AL or DR conditions for 96 hr are shown. Scale bars are 20 μm. I) The average proportion of worms with at least one embryo <i>in utero</i> is plotted against time in culture (starting from L1 arrest). Data are from sampling 5 independent biological replicates. J) The number of offspring laid after 96 hr in AL or DR culture is plotted (*p = 0.02, paired t-test, n = 3). Data are pooled and presented as a boxplot reflecting the quartiles. Whiskers extend to the lowest and highest data points within 1.5x the interquartile range. Outliers appear as dots, and diamonds depict the mean value of the pooled data.