Dynamics of HsbD and FhlF cellular (co-)localization.

<p><b>(A)</b> Localization of HsbD-YFP (green) and FhlF-RFP (red) in a <i>P</i>. <i>aeruginosa</i> wild-type strain. Scale bar = 2 μm. Bottom panel: three representative patterns (1:1, 1:2 foci and 2:2 YFP:RFP foci) are illustrated in a closer view. Scale bar = 1 μm. Cell boundaries are delineated in white. <b>(B)</b> HsbD and FhlF polar localization frequency and their colocalization. More than 200 cells were analyzed from different fields. At least three independent experiments were performed. <b>(C)</b> Dynamics of HsbD-YFP (green) and FhlF-RFP (red). An overlay of the fluorescence channel(s) and the phase contrast image illustrate HsbD-YFP and FlhF-RFP subcellular localization pattern. Fluorescence images are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006354#pgen.1006354.s006" target="_blank">S6 Fig</a>. Scale bar = 1 μm. Bottom panel shows the quantification of the HsbD-YFP (green) and FhlF-RFP (red) fluorescence intensity across the cell length. Cartoon: representation of coordinates reported in the graph.