The B-1 biased fate of FL-CLPs is stable in culture.

<p>(A) Absolute numbers of B-1a, B-1b and B-2 cells in the SCID mice transferred with cells from cultures. FL-CLPs or 8-wk-ABM-CLPs were cultured on ST2 for 6 days and 4.0–5.0 x10⁴ cells were transferred to irradiated SCID mice. 3 weeks later, peritoneal and spleen cells were analyzed as described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0165676#pone.0165676.g001" target="_blank">Fig 1B</a>. (B) Surface markers of day6 ST2 cultures initiated with FL-CLPs or ABM-CLPs. The data are representative of two independent experiments with similar results (n = 5). (C) Expression of MHC II on the day6 ST2 cultures initiated with FL-CLPs or ABM-CLPs with or without IL-7. (*<i>P</i> < 0.05)