Chronic infection model mice show increased splenic Tregs over the time course of WNV infection compared to mice with disease.

<p>(A) Three representative RIX lines with disease are compared to the chronic line, with T cell subsets and activation markers measured by flow cytometry as indicated (blue = up, red = down); data from spleen. (B) CD29+ Treg frequencies in the spleen. At d7 post-infection, differences in spleen CD29+ Treg frequency were statistically significant for CC(032x013)F1 compared to CC(005x001)F1, CC(061x026)F1, and CC(016x038)F1 (p<0.005). (C) Flow cytometry analysis heat maps from brain samples for the same lines and time points. (D) Brain Treg numbers. N = 3 mice per group for all time points shown.