Δ<i>yvcK</i> mutants phenocopy Δ<i>prkA</i> mutants <i>in vitro</i>, <i>ex vivo</i> and <i>in vivo</i>.

<p><b>(A</b>) Growth of the Δ<i>yvcK</i> mutant in BHI, <b>(B)</b> or in IMM with glucose-6-phosphate (filled symbols and solid lines) or glycerol (open symbols and dashed lines) as the sole carbon source. Data are averages of three biological replicates and error bars represent standard deviation of the mean. <b>(C)</b> Morphology of wild-type and the Δ<i>yvcK</i> mutant in BHI, IMM Glucose-6-phosphate, and IMM Glycerol at OD 0.5 (BHI) or 6 hours post inoculation. <b>(D)</b> Intracellular growth of wild-type (black circles) and Δ<i>yvcK</i> mutants (red squares) was determined in bone marrow-derived macrophages (BMDMs) following infection at an MOI of 0.2. <b>(E)</b> Intracellular lysis of <i>L</i>. <i>monocytogenes</i> was measured in immortalized IFNAR^-/- macrophages. Macrophages were infected at an MOI of 5 by <i>L</i>. <i>monocytogenes</i> strains carrying pBHE573 and macrophage luciferase expression was measured 6 hours post infection. <b>(D, E)</b> Mean values are reported. Experiments are representative of three or more biological replicates and error bars represent the standard deviation of technical triplicates. * indicates statistical significance by Student’s T-test (P<0.05). <b>(F)</b> Host Cell Death induced in wild-type C57/Bl6 BMDMs was analyzed by lactate dehydrogenase release 6 hours post infection at an MOI of 5. Data is an average of three biological replicates and error bars represent standard deviation of the mean. * indicates statistical significance by Student’s T-test (P<0.05). <b>(G)</b> C57Bl6 mice were infected intravenously with 1x10⁵ wild-type (black circles) or Δ<i>yvcK</i> mutants (red squares) <i>in vivo</i>. Spleens (left) and Livers (right) were harvested 48 hours post infection homogenized and plated for CFU. The median (solid bar) and limit of detection (line) for each experiment is indicated. Data are representative of two independent experiments with 5 mice each. * indicates statistical significance by Mann-Whitney Test (P<0.05).