RT-PCR verification of genes identified in transcriptome analysis.

<p>(A) RT-PCR analysis of 11 genes. (B) Comparison of relative gene expression in normal subjects and patients with ALS. Eleven genes from the Toll-like receptor and NOD-like receptor signaling pathways were verified using RT-PCR and genes—<i>CD14</i>, interferon-α/β receptor-1 (<i>IFNAR-1</i>), toll-interacting protein (<i>TOLLIP</i>), mitogen-activated protein kinase 9 (<i>MAPK9</i>), interleukin 1 beta (<i>IL-1β</i>), interleukin 8 (<i>IL-8</i>), and chemokine (C-X-C motif) ligand 1 (<i>CXCL1</i>)—were upregulated in the ALS group. *<i>P</i> < 0.05, ^†<i>P</i> < 0.1