BMP signal regulates <i>scrib</i> transcription in the pupal wing.

<p>(A) Scrib-GFP (green), pMad staining (red), and DAPI (blue) at 18 to 24 h AP. Nuclei are marked by DAPI (blue) staining. (B-E) Plot profile analysis of Scrib-GFP (green) and pMad (red) in ROIs in A, corresponding to 18h AP, 20h AP, 22h AP and 24h AP, respectively. (F) Quantitative PCR analysis of mRNA levels in pupal wings at 24 h AP. mRNA levels in <i>dpp</i>^<i>shv</i> <i>> ca-tkv</i> flies were compared to control <i>yw</i> flies. Relative mRNA expression level of genes of interest was calculated with normalization to <i>gapdh</i> mRNA level. <i>ventral veinless</i> (<i>vvl</i>) was used as a positive control. Error bar, SEM. ****<i>P</i><0.001, **<i>P</i><0.01, two-paired <i>t</i>-test. (G, H) In situ hybridization analysis of <i>scrib</i> (G) and <i>dlg1</i> (H) in pupal wings at 24 h AP (upper panels). caTkv expression clones were generated using MARCM. Clones were marked by GFP (lower panels). Up-regulated <i>scrib</i> or <i>dlg1</i> corresponding to caTkv clones are indicated by arrows. Sense probe was applied to the wings expressing caTkv clones as a negative control.