Practical applications of the staining method for analysis of longitudinal sections.

<p>A longitudinal section from a rat plantaris muscle was stained using the staining method. The whole image provides a global view of the MyHC isoforms present in the section. The magnified images in panels 1 and 2 show the presence of hybrid fibers comprised of MyHC2X (green) and MyHC2B (red), or of MyHC2A (blue) and MyHC2X (green), as indicated by yellow and red arrows, respectively. Bars indicate 1 mm in the whole image and 250 μm in the magnified images, respectively.