Analysis of TNF-α expression.

<p>FLS were treated exactly as described in the legend for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164207#pone.0164207.g002" target="_blank">Fig 2</a>. The conditioned media were collected from each group, and the concentration of TNF-α in the conditioned media was estimated by ELISA. *, comparison with the control group, <i>p</i><0.05; ^#, comparison with the IL-1β group, <i>p</i>< 0.05.