Pairs of mutants with transposon insertions in the same gene.

<p>(A) Locations of transposon insertion sites. In the pool of mutants used to challenge the animals, we included three pairs of mutants that have insertion sites at different locations in the same gene. The location of both transposon insertions is designated by black arrows in each of the following genes: <i>lic12324</i> (insertion sites: 2806250 and 2806718 in the Fiocruz L1-130 genome), <i>lic12327</i> (insertion sites 2810027 and 2810271) and <i>lic12627</i> (insertion sites: 3177595 and 3178505). (B-G) Fitness of each pair of mutants. The output/input ratios for each of the eight animals were determined for each mutant in blood, kidney and liver. Each ratio is represented by a black diamonds and the median of these ratios by a red line. The dotted line represents fitness of 1.0, which means neutral fitness. The median was compared to 1.0 using the Wilcoxon rank test. The number of asterisks indicates the significance level: * P < 0.05. No statistically significant differences were observed between the pair of <i>lic12327</i> mutants or between the <i>lic12324</i> mutants for any tissue. The comparison of the ratios of these two <i>lic12627</i> mutants confirmed statistically significant differences in kidney (P = 0.0379) and liver (P = 0.0011).