Detection limit of RT-LAMP and RT-PCR assay using total RNA extracts.

<p>Extracted total RNA from a <i>P</i>. <i>falciparum</i>-infected blood sample was serially diluted (10³to 10^-3ng/μL) and 2.5μL tested by both RT-LAMP and RT-PCR methods. <b>A)</b> Agarose (1.2%) gel analyses and visualization of DNA laddering in RT-LAMP amplified samples. <b>B)</b> Agarose (1.2%) gel analyses of the <i>PfExp1</i> RT-PCR products, showing similarities in the detection limits of both RT-LAMP and RT-PCR methods. <i>Mk</i>: 1Kb DNA ladder, <i>NTC</i>: no-template control reaction tube, and numbers on each lane indicate the total RNA concentration (in ng/μL) in each tube prior to the assays.<b>C)</b> Linear regression analyses showing a significant inverse correlation between total RNA amounts and the time-to-peak amplification signal for the RT-LAMP method. Data is representative ofthe lowest limit of detection obtained from three replicate experiments at different diluted RNA concentration ranges.