K1 and AMPK association is important for cell survival following exposure to stress.

<p>(A) HEK-293 cells stably expressing FLAG-tagged K1_WT, K1_ΔCT, K1_ΔNT or empty vector (EV = pLenti CMV) were treated with 10 or 7.5 μM compound C for 48 hours. The percent of metabolically active cells was determined using the Promega MTS assay. The percent was derived by normalization to the DMSO (0.1%) control. Error bars represent the standard deviation of biological triplicates. GraphPad Prism was used to determine one-way ANOVA and Tukey’s post-test. **<i>P</i><0.005 (B) Western blot showing expression of EV, K1_WT, K1_ΔCT, and K1_ΔNT using an anti-FLAG antibody. (C) Western blot from (Fig 7B) was stripped and probed for K1 using an anti-K1 antibody.